Stem Cell Reports Report Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain

Neural stem/progenitor cells (NSPCs) generate new neurons throughout life in distinct areas of the adult mammalian brain. Besides classical transgenesis-based approaches, retrovirus-mediated genetic manipulation is frequently used to study mechanisms that regulate neurogenesis in the nervous system. Here, we show that fusion of a tamoxifen-regulatable estrogen receptor (ER(T2)) motif to transcription factors (i.e., ASCL1 and NEUROD1) enables temporal control of transgene expression in adult mouse NSPCs in vitro and in vivo. Thus, the approach described here represents a versatile strategy for regulating gene expression to study gene function in dividing cells and their progeny. DOI: https://doi.org/10.1016/j.stemcr.2013.06.003 Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-86130 Published Version Originally published at: Braun, Simon M G; Machado, Raquel A C; Jessberger, Sebastian (2013). Temporal control of retroviral transgene expression in newborn cells in the adult brain. Stem Cell Reports, 1(2):114-122. DOI: https://doi.org/10.1016/j.stemcr.2013.06.003 Stem Cell Reports Report Temporal Control of Retroviral Transgene Expression in Newborn Cells in the Adult Brain Simon M.G. Braun,1,2,3 Raquel A.C. Machado,1,2 and Sebastian Jessberger1,2,3,* 1Brain Research Institute, Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland 2Institute of Molecular Health Sciences, Department of Biology, Swiss Federal Institute of Technology (ETH) Zurich, 8093 Zurich, Switzerland 3Neuroscience Center Zurich, University of Zurich and ETH Zurich, 8057 Zurich, Switzerland *Correspondence: [email protected] http://dx.doi.org/10.1016/j.stemcr.2013.06.003 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.